Preparing
Whole blood Genomic DNA Extraction/Isolation Kit
Rapid and reliable purification of high-quality genomic DNA from whole blood, PBMCs
Product Description
The Whole Blood Genomic DNA Extraction Kit is designed for the rapid and efficient purification of high‑quality genomic DNA from up to 200 μL of whole blood, peripheral blood mononuclear cells (PBMCs), and dried blood spots.
Genomic DNA purification is based on silica membrane spin‑column technology, eliminating the need for hazardous phenol/chloroform extraction, time‑consuming alcohol precipitation, or expensive resin‑based purification methods.
Spin columns selectively bind DNA under optimized high‑salt conditions. Following the removal of contaminants through washing steps, the bound DNA is efficiently eluted under low‑salt, mildly alkaline conditions.
Following cell lysis, the Main Protocol can be completed in less than 30 minutes and yields purified genomic DNA with fragment sizes greater than 30 kb.
The purified genomic DNA is suitable for direct use in a wide range of downstream applications, including PCR, qPCR, Southern blotting, and enzymatic reactions.
Contents
| KIT COMPONENTS | 250 Tests OttoP9510‑250 | 100 Tests OttoP9510‑100 | 50 Tests OttoP9510‑50 |
| Lysis Solution (LBD) | 70 mL | 27 mL | 14 mL |
| Proteinase K Solution (PKD) | 5 × 1.25 mL | 2 × 1.25 mL | 1.25 mL |
| Wash Solution 1 (WBD‑1) | 85 mL | 34 mL | 17 mL |
| Wash Solution 2 (WBD‑2) | 85 mL | 33 mL | 17 mL |
| Spin Columns and Collection Tubes | 250 | 100 | 50 |
| Elution Solution (EBD) | 25 mL | 15 mL | 15 mL |
About the Otto Scientific product ecosystem
Develops biomarker-focused kit families for scientific research and translational studies.
Produces ELISA-oriented product groups for routine research and assay development use.
Designs oxidative stress panels for Total Antioxidant Status (TAS), Total Oxidant Status (TOS), Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx), and Catalase workflows.
Builds reagent structures compatible with cell culture studies and MTT assay workflows.
Supports western blot analysis and real-time PCR applications with assay-specific components and documentation.
Extends product planning for pathology, histopathology, immunohistochemistry, and selected trace element analysis studies.
Prepares product documents, datasheets, and certificates in a clear structure for scientific teams.
Maintains batch continuity, packaging discipline, and repeat order consistency across product families.
Aligns product combinations with academic and industrial research program requirements.
Shares raw material, lead time, and supply planning information transparently.
Provides product communication for storage, setup, and application-specific usage questions.
Supports manufacturer-direct communication from first review to repeat procurement steps.
Brief Information About Otto Scientific
- Developing test kits and consumables for biochemistry, molecular biology, pathology, and cell culture workflows.
- Managing technical documentation, traceability, and quality records through structured product processes.
- Supporting universities, R&D centers, and industrial teams with manufacturer-direct product communication.
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Otto Scientific
Scientific products for research teams
We develop qPCR, ELISA, western blot, immunohistochemistry, and oxidative stress-compatible product families for research and diagnostic teams.
Our portfolio is built around repeatability, documentation clarity, and practical application use.
