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ALP Assay Kit
The ALP Test Kit is designed for the quantitative determination of alkaline phosphatase activity. Cat.No: OttoBC124
Product Description
Alkaline phosphatase in serum consists of four structural genotypes: the liverbone‑ kidney type, the intestinal type, the placental type and the variant from germ cells. It occurs in osteoblasts, hepatocytes the kidneys, spleen, placenta, prostate, leukocytes and the small intestine. The liver‑bone‑kidney type is particularly important. A rise in the alkaline phosphatase activity occurs with all forms of cholestasis, particularly with obstructive jaundice. It is also elevated in diseases of the skeletal system, such as Pagefs disease, hyperparathyroidism, rickets and osteomalacia, as well as with fractures and malignant tumors. A considerable rise in the alkaline phosphatase activity is sometimes seen in children and juveniles. It is caused by increased osteoblast activity following accelerated bone growth. Various reference values for the purposes of clinical evaluation have been assigned to different age groups. In 1946, Bessey, Lowry and Brock published a method for the determination of alkaline phosphatase using p‑nitrophenyl phosphate as substrate buffered with glycine/NaOH. In 1967, Hausamen et al improved upon the method by using diethanolamine as buffer. The "optimized standard method" by using diethanolamine as buffer. The assay described here meets the recommendations of the IFCC.
Contents
Colorimetric assay in accordance with a standardized method.
p ‑ Nitrophenylphosphate+ H2OPhosphate + p ‑ Nitrophenol
In the presence of magnesium and zinc ions, p‑nitrophenyl phosphate is hydrolyzed by phosphatases to form phosphate and p‑nitrophenol. In this process AMP serves as transient phosphate acceptor. The release of coloured pnitrophenol is proportional to the ALP activity and can be measured photometrically
Referanslar
1. Bessey OAH et al. J Biol Chem 1946;164:321.
2. Empfehlungen der Deutschen Gesellschaft für Klinische Chemie. StandardMethode zur Bestimmung der Aktivität der alkalischen Phosphatase. Z klin Chem u klin Biochem 1972;10:191.
3. Glick MR, Ryder KW, Jackson SA. Graphical Comparisons of Interferences in Clinical Chemistry Instrumentation. Clin Chem 1986;32:470‑474.
4. Greiling H, Gressner AM (Hrsg.). Lehrbuch der Klinischen Chemie und Pathobiochemie, 3rd . Stuttgart/New York: Schattauer Verlag, 1995.
5. Guder WG, Narayanan S, Wisser H, Zawta B. List of Analytes Preanalytical Variables. Broschüre in: Samples: From the Patient to the Laboratory. Darmstadt: GIT Verlag,l996.
6. Hausamen TU et al. Clin Chim Acta 1967;15:241.
7. Rosalki SB, Foo AY, Burlina A et al. Multicenter Evaluation of Iso ALP Test Kit for Measurement of Bone Alkaline Phosphatase Activity in Serum and Plasma. Clin Chem 1993;39:648‑652.
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