Preparing
ALT Assay Kit
The ALT Test Kit is intended for the quantitative determination of alanine aminotransferase (ALT) activity. Cat.No: OttoBC128
Product Description
Alanine aminotransferase (glutamate‑pyruvate‑transaminase) belongs to the group of transaminases which catalyze the conversion of amino acids to the corresponding a‑keto acids via the transfer of amino groups; they also catalyze the reverse process. Although higher activities exist in the liver, minor activity can also be detected in the kidneys, heart, skeletal muscle, pancreas, spleen, and lungs. Elevated levels of transaminases are indicative of myocardial infarction, hepatopathies, muscular dystrophy, and damage to internal organs. Increased ALT activity in the serum, however, is a rather specific indicator of damage to the liver parenchyma, while AST is not necessarily a liver‑specific parameter. The International Federation of Clinical Chemistry (IFCC) recommended standardized methods for the determination of ALT with optimized substrate concentrations, use of TRIS buffer, simultaneous preincubation of serum with buffer (to avoid competing reactions with NADH), substrate start, and pyridoxal phos‑phate activation. The method described here is derived from the IFCC reference method.
Contents
UV test according to the IFCC method.
L‑Alanin + 2‑Oxoglutarate <⎯⎯> L‑Glutamate + Pyruvat
Pyruvat + NADH + H + <⎯⎯> D‑Lactate+ NAD+
The enzyme alanine aminotransferase (EC 2.6.1.2; L‑Alanine:2‑Oxoglutarate Aminotransferase, ALT or A1aAT; Glutamate Pyruvate Transaminase, GPT) catalyzes the tran‑ saminase reaction between L‑Alanine and 2‑Oxoglutarate. The pyruvate formed, is reduced to lactate in the presence of LDH. As the reactions proceed, NADH is oxidized to NAD+ . The disappearance of NADH per unit time is followed by measuring the decrease in absorbance at 340 nm.
Referanslar
1.J. Clin.Chem.Clin.Biochem 8 (1970) 658; 10 (1972) 182
2.Tietz Textbook of Clinical Chemistry, Second Edition, Burtis‑Ashwood (1994).
3. HU Bergmeyer ‑ Methods of enzymatic analysis, (1987). CCLM 2002; 40(7):725‑733, Schumann et al.
4. IFCC reference procedure for aspartate aminotransferase.
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