Preparing
Albumin Assay Kit
This Albumin Test Kit is based on a colorimetric endpoint method using bromocresol green (BCG). Cat.No: OttoBC123
Product Description
Albumin is a carbohydrate‑free protein, which constitutes 55‑65% of total plasma protein. It maintains oncotic plasma pressure, is also involved in the transport and storage of a wide variety of ligands and is a source of endogenous amino acids. Albumin binds and solubilized various compounds, e.g. bilirubin, calcium and long chain fatty acids. Furthermore albumin is capable of binding toxic heavy metals ions as well as numerous pharmaceuticals, which is the reason why lower albumin concentrations in blood have a significant effect on pharmacokinetics. Hyperalbuminemia is of little diagnostic significance except in the case of dehydration. Hyperalbuminemia occurs during many illnesses and is caused by several factors: Compromised synthesis due either to liver disease or as a consequence of reduced protein uptake, elevated catabolism due to tissue damage (severe burns) or inflammation, malabsorption of amino acids (Crohn's disease), proteinuria as a consequence of nephrotic syndrome; protein loss via the stool (neoplastic disease). In several cases of hyperalbuminemia, the maximum albumin concentration of plasma is 2.5g/dl. Due to the low osmotic pressure of the plasma water permeates through blood capillaries into tissue (edema). The determination of albumin allows monitoring of a controlled patient dietary supplementation and serves also as an excellent test of liver function.
Contents
Test Principle
Colorimetric assay, endpoint method
• Sample and addition of R1
• Start of the reaction
At a pH value of 4.1 albumin displays a sufficiently cationic character to be able to bind with bromocresol green (BCG), an anionic dyestuff, to form a blue‑green complex.
albumin + BCG → albumin BCG‑complex
The color intensity of the blue‑green color is directly proportional to the albumin concentration and can be determined photometrically.
Referanslar
1. Doumas B.T., Watson W.A., Biggs H.G.. Albumin standards and the measurement of serum albumin with bromocresol green. Clin Chim Acta 1971 ;31 :87‑96.
2. Glick M.R., Ryder K.W., Jackson SA. Graphical Comparisons of Interferences in Clinical Chemistry Instrumentation. Clin Chem 1986;32:470‑474.
3. Grant G.H., Silverman L.M., Christenson R.H.. Amino acids and proteins. In: Tietz N.W. (ed.). Fundamentals of Clinical Chemistry, 3rd Philadelphia, Pa: W.B. Saunders, 1987:328‑330.
4. Marshall WJ (ed.). Illustrated Textbook of Clinical Chemistry,3rd . London: Gower Medical Publishing, 1989:207‑218.
5. Tietz NW (ed.). Clinical Guide to Laboratory Tests, 3rd . Philadelphia, Pa: WB Saunders, 1895:22‑24
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- Developing test kits and consumables for biochemistry, molecular biology, pathology, and cell culture workflows.
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