Preparing
BILIRUBIN DIRECT Assay Kit
The Direct Bilirubin Test Kit is intended for the quantitative determination of direct (conjugated) bilirubin. Cat.No: OttoBC131
Product Description
80‑85% of bilirubin originates on degradation of hemoglobin with the other 15‑20% being derived from cytochrome, myoglobin and catalases. Unconjugated bilirubin, which binds to plasma albumin, is produced in the course of degradation in the reticuloendothelial system, liver Kupffer cells, spleen and bone marrow.Unconjugated (primary indirect, water‑insoluble) bilirubin is soluble in lipids. With the aid of the glucuronyl transferase enzyme, bilirubin is conjugated primarily by glucuronic acid in the microsomes of hepatic parenchymal cells. In contrast to unconjugated bilirubin, conjugated (secondary, direct) bilirubin is soluble in water, and is excreted via the kidneys. Bilirubin assays are suitable for evaluating the degree of severity of icteric clinical symptoms as well as for monitoring and objectively assessing these symptoms. Distinguishing between direct and indirect bilirubin is a valuable aid in the differential diagnosis of different forms of jaundice. A direct bilirubin value of 50% in hepatic and posthepatic jaundice.
Contents
Jendrassik‑Gróf method In the presence of caffeine accelerator, total bilirubin couples with sulfanilic acid to form a red azobilirubin dye, the color intensity which is proportional to the bilirubin concentration. Determination of direct bilirubin is performed without caffeine additive. The addition of alkaline tartrate causes a transformation from the red azobilirubin dye to a blue dye and the absorbance maximum from 546nm to 578nm.
Referanslar
1. Jendrassik L et al. Biochem Z 1938 ;297 :81.
2. Malloy HT, Evelyn KA. J Biol Chem 1937;119:481.
3. Richterin R. Klinische Chemie, 3. Auflage Basel: Karger Verlag, 1971:450‑
4. Schellong G, Wende U. Technik für die Eichung von Methoden zur Serumbilirubin‑Bestimmung. Klin Wschr 1960;38:703.
5. Schmidt E, Schmidt FW. Diagnostik des Ikterus. Dt med Wschr 1984;109:139‑ 144
6. Sherlock S. Liver Disease. London: Chuchill, 1951:204.
7. Silbernagel S, Despopoulus A. Physiologie für die Praxis, Medical 1983; 4 ; 492 – 495
8. Stiehl A. Hyperbilirubinämie bei Lebererkrankungen. Fortschr Med 1982; 100:842‑845
9. Thaler H. Bilirubin: Ein Wegwerfprodukt bleibt weiter aktuell. Dt. med Wschr 1984; 109:283 ‑ 284
10. Thomas L. Labor und Diagnose, Marburg: Med Verlagsges, 1984
About the Otto Scientific product ecosystem
Develops biomarker-focused kit families for scientific research and translational studies.
Produces ELISA-oriented product groups for routine research and assay development use.
Designs oxidative stress panels for Total Antioxidant Status (TAS), Total Oxidant Status (TOS), Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx), and Catalase workflows.
Builds reagent structures compatible with cell culture studies and MTT assay workflows.
Supports western blot analysis and real-time PCR applications with assay-specific components and documentation.
Extends product planning for pathology, histopathology, immunohistochemistry, and selected trace element analysis studies.
Prepares product documents, datasheets, and certificates in a clear structure for scientific teams.
Maintains batch continuity, packaging discipline, and repeat order consistency across product families.
Aligns product combinations with academic and industrial research program requirements.
Shares raw material, lead time, and supply planning information transparently.
Provides product communication for storage, setup, and application-specific usage questions.
Supports manufacturer-direct communication from first review to repeat procurement steps.
Brief Information About Otto Scientific
- Developing test kits and consumables for biochemistry, molecular biology, pathology, and cell culture workflows.
- Managing technical documentation, traceability, and quality records through structured product processes.
- Supporting universities, R&D centers, and industrial teams with manufacturer-direct product communication.
Similar Products
Similar products are suggested automatically based on category and filter matches.
Otto Scientific
Scientific products for research teams
We develop qPCR, ELISA, western blot, immunohistochemistry, and oxidative stress-compatible product families for research and diagnostic teams.
Our portfolio is built around repeatability, documentation clarity, and practical application use.
