Preparing
Catalase (CAT) Assay Kit
Catalase (CAT) is an important antioxidant enzyme that plays a key role in the body's defense against oxidative stress. It catalyzes the decomposition
Product Description
Catalase (CAT) catalyzes the decomposition of hydrogen peroxide (H₂O₂) into water (H₂O) and molecular oxygen (O₂). In this colorimetric assay, catalase present in the sample consumes a defined amount of hydrogen peroxide during the enzymatic reaction.
The residual hydrogen peroxide reacts with a chromogenic reagent to produce a colored compound. Therefore, higher catalase activity results in lower residual hydrogen peroxide and a corresponding change in color intensity.
Catalase activity can be determined by measuring the optical density (OD) at the specified wavelength and calculating the amount of hydrogen peroxide decomposed by catalase relative to the control.
Referanslar
1. Góth L. A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta. 1991;196(2‑3):143‑151. doi:10.1016/0009‑8981(91)90067‑M.
2. Koroliuk MA, Ivanova LI, Maiorova IG, Tokarev VE. A method of determining catalase activity. Lab Delo. 1988;(1):16‑19. PMID:2451064.
3. Aebi H. Catalase in vitro. Methods Enzymol. 1984;105:121‑126. doi:10.1016/S0076‑6879(84)05016‑3.
4. Beers RF Jr, Sizer IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem. 1952;195(1):133‑140. doi:10.1016/S0021‑9258(19)50881‑X.
5. Chance B, Maehly AC. Assay of catalases and peroxidases. Methods Enzymol. 1955;2:764‑775. doi:10.1016/S0076‑6879(55)02300‑8.
6. Sinha AK. Colorimetric assay of catalase. Anal Biochem. 1972;47(2):389‑394. doi:10.1016/0003‑2697(72)90132‑7.
7. Johansson LH, Borg LA. A spectrophotometric method for determination of catalase activity in small tissue samples. Anal Biochem. 1988;174(1):331‑336. doi:10.1016/0003‑2697(88)90554‑4.
About the Otto Scientific product ecosystem
Develops biomarker-focused kit families for scientific research and translational studies.
Produces ELISA-oriented product groups for routine research and assay development use.
Designs oxidative stress panels for Total Antioxidant Status (TAS), Total Oxidant Status (TOS), Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx), and Catalase workflows.
Builds reagent structures compatible with cell culture studies and MTT assay workflows.
Supports western blot analysis and real-time PCR applications with assay-specific components and documentation.
Extends product planning for pathology, histopathology, immunohistochemistry, and selected trace element analysis studies.
Prepares product documents, datasheets, and certificates in a clear structure for scientific teams.
Maintains batch continuity, packaging discipline, and repeat order consistency across product families.
Aligns product combinations with academic and industrial research program requirements.
Shares raw material, lead time, and supply planning information transparently.
Provides product communication for storage, setup, and application-specific usage questions.
Supports manufacturer-direct communication from first review to repeat procurement steps.
Brief Information About Otto Scientific
- Developing test kits and consumables for biochemistry, molecular biology, pathology, and cell culture workflows.
- Managing technical documentation, traceability, and quality records through structured product processes.
- Supporting universities, R&D centers, and industrial teams with manufacturer-direct product communication.
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Otto Scientific
Scientific products for research teams
We develop qPCR, ELISA, western blot, immunohistochemistry, and oxidative stress-compatible product families for research and diagnostic teams.
Our portfolio is built around repeatability, documentation clarity, and practical application use.
