Oxidative Stress Instrument

Antioxidant/Oxidant Balance Assays (Oxidative Stress)

0 out of 5

$120.00

Summary & Explanation
Free radicals are metabolic by products which are formed during energy conversion of nutrients in our bodies by using oxygen. Free radicals
(reactive oxygen species) are in unstable form and in order to become stable they attack and damage cells. Antioxidants neutralize free radicals to
protect cells from damage. Free radicals and antioxidants must be in balance thus antioxidants neutralize free radicals. If free radical level increased
against antioxidant level oxidative damage occurs and this state is called oxidative stress.
Oxidative stress is not a disease, but it is a factor that can cause or acelerate the disease. Genarally it is an important stimulus for preventive health
measures. But the dangerous is oxidative stress is absence of any symptoms. If this condition is not diagnosted and treated, could result serious
health problems and diseases. Exposure to toxins or pathogens, poor antioxidant defense system, irregular life style, extreme intense exercise and
daily metabolic products, causes oxidative stress.
Category: Oxidative Stress

Description

 

Overview

Oxidative stress

Total Antioxidant Status (TAS) Assay

TAS Test Principle
Antioxidants in the sample reduce dark blue-green colored ABTS
radical to colorless reduced ABTS form. The change of absorbance
at 660 nm is related with total antioxidant level of the sample. The
assay is calibrated with a stable antioxidant standard solution which
is traditionally named as Trolox Equivalent that is a vitamin E analog .

 

Content Explanation Shelf life
Reagent-1 1x30ml 12 months
Reagent-2 1x8ml 12 months
Calibrator 1×3ml 12 months
Q.Control Level-1 1×3ml 12 months
Q.Control Level-2 1×3ml 12 months
Total Oxidant Status (TOS) Assay

TOS Test Principle
Oxidants present in the sample oxidize the ferrous ion–chelator
complex to ferric ion. The oxidation reaction is prolonged by enhancer
molecules, which are abundantly present in the reaction medium. The
ferric ion makes a colored complex with chromogen in an acidic
medium. The color intensity, which can be measured
spectrophotometrically, is related to the total amount of oxidant
molecules present in the sample. The assay is calibrated with
hydrogen peroxide and the results are expressed in terms of
micromolar hydrogen peroxide equivalent per liter (μmol
H2O2Equiv./L)

 

Content Explanation Shelf life
Reagent-1 1x30ml 12 months
Reagent-2 1x8ml 12 months
Calibrator 1×3ml 12 months
Q.Control Level-1 1×3ml 12 months
Q.Control Level-2 1×3ml 12 months
Instrument
Oxidative Stress Instrument
Free radicals
References
1. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004 Apr;37(4):277-85.
2. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005 Dec;38(12):1103-11.

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