$120.00
Summary & Explanation
The MDA level was determined by a method based on the reaction with thiobarbituric acid (TBA) at 90–100 °C In the TBA test reaction, MDA or MDA-like substances and TBA react with the production of a pink pigment with a maximum absorption at 532 nm. The reaction was performed at pH 2–3 at 90_C for 15 min. The sample was mixed with two volumes of cold 10% (w/v) trichloroacetic acid for the precipitation of protein. The precipitate was pelleted by centrifugation, and an aliquot of the supernatant was reacted with an equal volume of 0.67% (w/v) TBA in a boiling water bath for 10 min. After cooling, the absorbance was read at 532 nm.
| CATALOG # | Otto1001 |
| PRODUCT NAME | Malondialdehyde (MDA) Assay Kit |
| APPLICATIONS | Spectrophotometer |
| REACTIVITY | Universal |
| SAMPLES TYPE | Serum, Plasma,Tissue homogenates, Biological |
| Content | Explanation | Shelf life |
| Reagent-1 | 1x40ml | 6 months |
| Reagent-2 | 1×20ml | 6 months |
| Calibrator | 1×0.5ml | 6 months |
References
Janero, D.R. Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury, Free Rad. Biol. Med. 9:515-540; 1990
