Creatine Kinase (CK) Assay Kit

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$120.00

Summary:

Creatine kinase (CK) is a dimeric enzyme occurring in four different forms: mitochondrial isoenzyme and the cytosolic isoenzymes CK-MM (muscle type), CKBB (brain type) and CK-MB (myocardial type). The determination of CK and CK-isoenzyme activities is utilized in the diagnosis and monitoring of myocardial infarction and myopathies such as the progressive Duchenne muscular dystrophy. Following injury to the myocardium, such as occurs with acute myocardial infarction. CK is released from the damaged myocardial cells. In early cases, a rise in the CK activity can be found just 4 hours after an infarction, the CK-activities reaches a maximum after 12-24 hours and then falls back to the normal range after 3-4 days. Myocardial damage is very likely when the total CK activity is above 190 U/l, the CK-MB activity is above 24 U/l (+37°C) and the CK-MB activity fraction exceeds 6% of the total. The assay method using creatine phosphate and ADP was first described by Oliver, modified by Rosalki and further improved for optimal test conditions by Szasz. CK is rapidly inactivated by oxidation of the sulfhydryl groups in the active center. The enzyme can be reactivated by the addition of acetylcysteine (NAc). Interference by adenylate kinase is prevented by the addition of diadenosine pentaphosphate and AMP. Standardized methods for the determination for CK using the “reverse reaction” and activation by NAc were recommended by the German Society for Clinical Chemistry (DGKC) and the International Federation of Clinical Chemistry (IFCC) in 1977 and 1990 respectively. This assay meets the recommendations of the IFCC and DGKC (Standard method 94).

Category: Colorimetric Kits

Description

 

Overview
CATALOG # OttoBC136
PRODUCT NAME Creatine Kinase (CK) Assay Kit
APPLICATIONS Fully automated analyzer, Spectrophotometer
REACTIVITY Universal
SAMPLES TYPE Serum, Plasma (Edta or Heparin)
Kit Components
Content Explanation Shelf life
Reagent-1 1x30ml 6 months
Reagent-2 1x8ml 6 months
Calibrator 1×0.5ml 6 months
Q.Control 1×0.5ml 6 months
References
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  4. Horder M., Elser R.C., Gerhard M. et al. Approved Recommendation on IFCC Methods for the Measurement of Catalytic Concentration Chem. Clin Biochem 1991;29:435-456
  5. Klauke R., Schmidt E., Lorentz K., Recommendations for carrying out standard ECCLS procedures (1988) for the catalytic concentration of creatine

kinase, aspartate aminotransferase, alanine aminotransferase and yglutamyltransferase at 37°C. Eur. J Clin Chem. Clin Biochem. 1993;31:901-909

  1. Lorentz K., Röhle G., Siekmann L. DG Klinische Chemie Mitteilungen 1995;26:190-192
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  1. Oliver I.T. Biochem J Lab Clin Chem. Med 1967; 69-696J. Clin. Chem. Clin. Biochem. 15, 249 (1977)
  2. Recommendations of the German society for Clinical chemistry. standardization of methods for the estimation of enzyme activities in biological

fluids: Standard method for the determination of creatine kinase activity. J Clin Chem Biochem 15: 255 -260 1977

  1. Stein W. Laboratory Diagnosis of Acute Myocardial Infarction. Darmstadt: GIT Verlag 1988: 34-37.
  2. Stein W. Med. Welt 1985;36:572
  3. Szasz G. Busch E.W. Third European Congress of Clinical Chemistry Brighton, England 3.-8. June 1979 (Abstract)
  4. Szasz, G. et al Clin. Chem. 22, 650 (1976)
  5. Thomas L. ed. Labor und Diagnose, 4th . Marburg: die Medizinische Verlagsgesellschaft.1992
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Saunders Company, 1995:486–487.

  1. Thomas L, Klein G. Neue vorläufige Normalbereiche für neun Serumenzyme.

Deutsches Ärzteblatt 2006;103;Heft 7

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