Description
| CATALOG # | OttoAB123 |
| PRODUCT NAME | Caspase 3 Polyclonal Antibody |
| APPLICATIONS | WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC) |
| REACTIVITY | Human, Mouse, Rat |
| PREDICTED REACTIVITY | Dog, Rabbit |
| CONJUGATION | Unconjugated |
| HOST | Rabbit |
| SOURCE | KLH conjugated synthetic peptide derived from rat Caspase 3 |
| IMMUNOGEN RANGE | 11-120/277 |
| CLONALITY | Polyclonal |
| ISOTYPE | IgG |
| CONCENTRATION | 1ug/ul |
| PURIFICATION | Purified by Protein A. |
| STORAGE BUFFER | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| STORAGE CONDITION | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| GENE ID | 25402 |
| SWISS PROT | P55213 |
| SUBCELLULAR LOCATION | Cytoplasm |
| SYNONYMS | Lice; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; IRP; Protein Yama; SREBP cleavage activity 1; SCA-1; Casp3; Cpp32 |
| BACKGROUND | Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a ‘216-Asp-|-Gly-217’ bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| FCM | 1:20-100 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |