Aspartate Aminotransferase (AST) (SGOT) Assay Kit

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$120.00

Summary & Explanation
Aspartate aminotransferase (glutamate-oxaloacetatetransaminase) belongs to the transaminases, which catalyze the interconversion of amino acids and a-keto
acids by transfer of amino groups. Aspartate aminotransferase is commonly found in human tissue. Although heart muscle is found to have the most
activity of the enzyme, significant activity has also been seen in the brain, liver, gastric mucosa, adipose tissue, skeletal muscle, and kidneys. AST is present in
both the cytoplasm and mitochondria of cells. In cases involving mild tissue injury, the predominant form of AST is that from the cytoplasm, with a smaller amount
coming from the mitochondria. Severe tissue damage results in more of the mitochondrial enzyme being released. Elevated levels of the transaminases can
signal myocardial infarction, hepatic disease, muscular dystrophy, and organ damage. The International Federation of Clinical Chemistry (IFCC)
recommended in 1977 and 1980 standardized procedures for AST determination, including optimization of substrate concentrations, employment
of TRIS* buffers, preincubation of combined buffer and serum to allow side reactions with NADH to occur, substrate start, and optional pyridoxal phosphate
activation. In 2002 the IFCC confirmed their recommendation and extend it to 37°C. This method is derived from the IFCC reference method. *TRIS = Tris(hydroxymethyl)-aminomethane

Category: Colorimetric Kits

Description

 

Overview
CATALOG # OttoBC127
PRODUCT NAME Aspartate Aminotransferase (AST) (SGOT) Assay Kit
APPLICATIONS Fully automated analyzer, Spectrophotometer
REACTIVITY Universal
SAMPLES TYPE Serum, Plasma (Edta or Heparin)
Kit Components
Content Explanation Shelf life
Reagent-1 1x30ml 6 months
Reagent-2 1x8ml 6 months
Calibrator 1×0.5ml 6 months
Q.Control 1×0.5ml 6 months
Analytical Performance

Inter Assay Coefficent of Variation (CV) % 1.21
Intra Assay Coefficent of Variation (CV) % 2.34

References

1. Bergmeyer HU, Herder M, Rej R. Approved recommendation (1985) on IFCC methods for the measurement of catalytic concentration of enzymes. Part 2.
IFCC Method for aspartate aminotransferase. J Clin Chem Clin Biochem 1986;24:49.
2. Glick MR, Ryder KW, Jackson SA. Graphical Comparisons of Interferences in Clinical Chemistry Instrumentation. Clin Chem 1 986;32:470-474.
3. Greiling H, Gressner AM (Hrsg.). Lehrbuch der Klinischen Chemie und Pathobiochemie,3. Auflage. Stuttgart/New York: Schattauer Verlag, 1995
4. Thefeld W et al. Dtsch med Wschr 1974;99:343.
5. Tietz NW (Hrsg.). Clinical Guide to Laboratory Tests, 3. Auflage. Philadelphia, PA: WB Saunders, 1995:76-77.
6. Wallnöfer H, Schmidt E, Schmidt FW (Hrsg.). Synopsis der Leberkrankheiten. Stuttgart: Georg Thieme Verlag, 1974.
7. Thomas L, Klein G. Neue vorläufige Normalbereiche für neun Serumenzyme. Deutsches Ärzteblatt 2006;103;Heft 7.
8. Schumann G et al. IFCC Primary Reference Procedures for the Measurement of Catalytic Activity Concentrations of Enzymes at 37°C – Part 4. Reference
Procedure for the Measurement of Catalytic Activity Concentrations of Alanine Aminotransferase. Clin Chem Lab Med 2002;40(7):718–724.
9. Thefeld W, Hoffmeister H, Busch, E-W, Koller PU, Vollmar J. Referenzwerte für die Bestimmungen der Transaminasen GOT und GPT sowie der
alkalischen Phosphatase im Serum mit optimierten Standardmethoden. Dtsch Med Wschr 1974;99:343-351.
10. Klein G, Lehmann P, Michel E, Regenauer H. Vergleich der IFCC-Methoden für ALAT, ASAT und GGT bei 37°C mit den eingeführten Standardmethoden
bei 25°C und 37°C. Lab Med 1994;18:403-404.

11. Wroblewski F LaDue JS. Ann Intern Med 1956;45:801.
12. Wroblewski F, LaDue JS. Proc Soc Exp Biol Med 1956;91 :569.

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