Alkaline Phosphatase (ALP) Assay Kit

Summary & Explanation
Aspartate aminotransferase (glutamate-oxaloacetatetransaminase) belongs to the transaminases, which catalyze the interconversion of amino acids and a-keto
acids by transfer of amino groups. Aspartate aminotransferase is commonly found in human tissue. Although heart muscle is found to have the most
activity of the enzyme, significant activity has also been seen in the brain, liver, gastric mucosa, adipose tissue, skeletal muscle, and kidneys. AST is present in
both the cytoplasm and mitochondria of cells. In cases involving mild tissue injury, the predominant form of AST is that from the cytoplasm, with a smaller amount
coming from the mitochondria. Severe tissue damage results in more of the mitochondrial enzyme being released. Elevated levels of the transaminases can
signal myocardial infarction, hepatic disease, muscular dystrophy, and organ damage. The International Federation of Clinical Chemistry (IFCC)
recommended in 1977 and 1980 standardized procedures for AST determination, including optimization of substrate concentrations, employment
of TRIS* buffers, preincubation of combined buffer and serum to allow side reactions with NADH to occur, substrate start, and optional pyridoxal phosphate
activation. In 2002 the IFCC confirmed their recommendation and extend it to 37°C. This method is derived from the IFCC reference method. *TRIS = Tris(hydroxymethyl)-aminomethane