GGT (Gamma GT) Assay Kit

Summary:
Gamma-glutamyltransferase (GGT) is used in the diagnosis and monitoring of he-patobiliary diseases. The enzymatic activity of GGT is often the only parameter with increased values when testing for such diseases, and is one of the most sen-sitive indicators. Gamma-glutamyltransferase is also a sensitive screening test for occult alcoholism. Elevated GGT activities are found in the serum of patients requiring long-term medication with phenobarbital and phenytoin. In 1969, Szasz published the first kinetic procedure for GGT in serum using y- glutamyl-p-nitro-anilide as substrate and glycylglycine as acceptor. In order to circumvent the poor solubility of Y-glutamyl-p-nitroanilide, Persijn and van der Silk investigated vari-ous derivatives of the compound with respect to solubility. The substrate L-Y-glutamyl-3-carboxy-4-nitroanilide is superior in terms of stability and solubility. The assay described beiow uses the water-soluble substrate L-Y-glutamyl-3- car-boxy-4-nitroanilide. The results correiate with those derived using the original substrate.